Consequential considerations when mapping tRNA fragments
نویسندگان
چکیده
منابع مشابه
Disrupted tRNA Genes and tRNA Fragments: A Perspective on tRNA Gene Evolution
Transfer RNAs (tRNAs) are small non-coding RNAs with lengths of approximately 70-100 nt. They are directly involved in protein synthesis by carrying amino acids to the ribosome. In this sense, tRNAs are key molecules that connect the RNA world and the protein world. Thus, study of the evolution of tRNA molecules may reveal the processes that led to the establishment of the central dogma: geneti...
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Motivation Many repetitive DNA elements are transcribed at appreciable expression levels. Mapping the corresponding RNA sequencing reads back to a reference genome is notoriously difficult and error-prone task, however. This is in particular true if chemical modifications introduce systematic mismatches, while at the same time the genomic loci are only approximately identical, as in the case of...
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A long-outstanding goal of protein engineering has run up against the tRNA world, a situation that, in turn, makes the achievement of that goal a formidable challenge. The goal is to expand the genetic code to allow for incorporation of additional, nonnatural, amino acids at predetermined sites in proteins that are synthesized in vivo. Achieving this goal would enable a variety of applications,...
متن کاملThe widespread occurrence of tRNA‐derived fragments in Saccharomyces cerevisiae
Short RNAs derived from the cleavage of tRNA molecules are observed in most organisms. Their occurrence seems to be induced by stress conditions, but still little is known about their biogenesis and functions. We find that the recovery of tRNA fragments depends on the RNA isolation method. Using an optimized RNA extraction protocol and northern blot hybridization technique, we show that the tRN...
متن کاملBeyond the Ribosome: Extra-translational Functions of tRNA Fragments.
High-throughput sequencing studies of small RNAs reveal a complex milieu of noncoding RNAs in biological samples. Early data analysis was often limited to microRNAs due to their regulatory nature and potential as biomarkers; however, many more classes of noncoding RNAs are now being recognized. A class of fragments initially excluded from analysis were those derived from transfer RNAs (tRNAs) b...
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ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2016
ISSN: 1471-2105
DOI: 10.1186/s12859-016-0921-0